Improved resolution of tryptic digest fragments from haemoglobin variants using phytic acid in free zone capillary electrophoresis.

doi:10.1002/bmc.1130080413

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Article

Improved resolution of tryptic digest fragments from haemoglobin variants using phytic acid in free zone capillary electrophoresis.

Biomedical chromatography : BMC - 1 Jan 2000

Okafo G N, Perrett D, Camilleri P

Abstract excerpt

Capillary electrophoresis can be applied to the rapid characterization of tryptic digests of proteins. The addition of phytic acid to the separation buffer was found to improve resolution considerably when the technique was applied to differentiate between tryptic digests derived from variant hae...

Topics

Animals
Chromatography, High Pressure Liquid
Chromatography, Paper
Electrophoresis
Genetic Variation
Hemoglobins
Humans
Peptide Fragments
Peptide Mapping
Phytic Acid

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