Article
Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum.
Journal of bacteriology - 1 Mar 1995
Kronemeyer W, Peekhaus N, Krämer R, Sahm H, Eggeling L
Abstract excerpt
To assess the mechanism and function of the glutamate uptake system of gram-positive Corynebacterium glutamicum, a mutant deficient in glutamate uptake was isolated and was then used to isolate a DNA fragment restoring this deficiency. In a low-copy-number vector, this fragment resulted in an increased glutamate uptake rate of 4.9 nmol/min/mg (wild type, 1.5 nmol/min/mg). In addition, carbon source-dependent...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Amino Acid Transport System X-AG
- Anti-Bacterial Agents
- Base Sequence
- Biological Transport
- Cloning, Molecular
- Corynebacterium
- Genes, Bacterial
- Genetic Complementation Test