Restriction enzyme-mediated DNA integration in Coprinus cinereus.

doi:10.1007/s004380050542

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Article

Restriction enzyme-mediated DNA integration in Coprinus cinereus.

Molecular & general genetics : MGG - 1 Sept 1997

Granado J D, Kertesz-Chaloupková K, Aebi M, Kües U

Abstract excerpt

Restriction enzyme-mediated DNA integration (REMI) has recently received attention as a new technique for the generation of mutants by transformation in fungi. Here we analyse this method in the basidiomycete Coprinus cinereus using the homologous pabI gene as a selectable marker and the restrict...

Topics

Blotting, Southern
Coprinus
DNA Restriction Enzymes
DNA, Fungal
Genetic Markers
Mutation
Plasmids
Transformation, Genetic

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