Article
Restriction enzyme-mediated DNA integration in Coprinus cinereus.
Molecular & general genetics : MGG - 1 Sept 1997
Granado J D, Kertesz-Chaloupková K, Aebi M, Kües U
Abstract excerpt
Restriction enzyme-mediated DNA integration (REMI) has recently received attention as a new technique for the generation of mutants by transformation in fungi. Here we analyse this method in the basidiomycete Coprinus cinereus using the homologous pabI gene as a selectable marker and the restriction enzymes BamHI, EcoRI and PstI. Addition of restriction enzymes to transformation mixtures results in an earlier...
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