Article
Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus.
Current genetics - 1 Mar 2004
Makino Rika, Kamada Takashi
Abstract excerpt
We identified 14 Coprinus cinereus variants defective in nuclear migration for dikaryosis after restriction enzyme-mediated integration mutagenesis. All the variants were able to donate nuclei but failed to accept nuclei in compatible matings. Of the 14 variants, six were due to single-gene mutations. We characterized num1, a gene responsible for one of the mutations. The num1 ORF interrupted by three introns is...
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