Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus.

doi:10.1007/s00294-003-0466-4

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Article

Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus.

Current genetics - 1 Mar 2004

Makino Rika, Kamada Takashi

Abstract excerpt

We identified 14 Coprinus cinereus variants defective in nuclear migration for dikaryosis after restriction enzyme-mediated integration mutagenesis. All the variants were able to donate nuclei but failed to accept nuclei in compatible matings. Of the 14 variants, six were due to single-gene mutations. We characterized num1, a gene responsible for one of the mutations. The num1 ORF interrupted by three introns is...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Cell Nucleus
Coprinus
DNA, Fungal
Diploidy
Down-Regulation
Fungal Proteins
Genes, Fungal
Genes, Mating Type, Fungal
Molecular Sequence Data
Mutagenesis
Mutation

Source

Open in PubMed

Identifiers

PMID: 14624309
DOI: 10.1007/s00294-003-0466-4

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