Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis.

Abstract excerpt

We have identified a phospholipase D gene (pld) fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant...

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