Article
A physical assay for meiotic recombination in Coprinus cinereus.
Molecular & general genetics : MGG - 28 Apr 1997
Freedman T, Pukkila P J
Abstract excerpt
We have used the polymerase chain reaction (PCR) method to monitor meiotic recombination in the basidiomycete Coprinus cinereus. We used DNA-mediated transformation to recover strains with modifications of the trp1 locus. The modifications were designed to introduce unique PCR priming sites separated by a homologous 2.4 kb region in which crossing over could occur. We showed that exchange occurred in this region...
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