pXen, a utility vector for the expression of GST-fusion proteins in Xenopus laevis oocytes and embryos.

doi:10.1016/s0378-1119(97)00171-6

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Article

pXen, a utility vector for the expression of GST-fusion proteins in Xenopus laevis oocytes and embryos.

Gene - 1 Sept 1997

MacNicol M C, Pot D, MacNicol A M

Abstract excerpt

We describe a plasmid, pXen, designed for the optimized expression of proteins fused to glutathione-S-transferase (GST) in Xenopus laevis oocytes and embryos. The Xenopus model system permits the biochemical analysis of signaling pathways and analysis of embryo phenotype in response to manipulati...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cyclic AMP-Dependent Protein Kinases
Embryo, Nonmammalian
Female
Gene Expression Regulation, Developmental
Genetic Vectors
Glutathione Transferase
Microinjections
Molecular Sequence Data
Mutation
Oncogene Proteins v-raf
Oocytes
RNA
Recombinant Fusion Proteins
Retroviridae Proteins, Oncogenic
Transcription, Genetic

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Identifiers

PMID: 9322737
DOI: 10.1016/s0378-1119(97)00171-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9322737-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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