Article
pXen, a utility vector for the expression of GST-fusion proteins in Xenopus laevis oocytes and embryos.
Gene - 1 Sept 1997
MacNicol M C, Pot D, MacNicol A M
Abstract excerpt
We describe a plasmid, pXen, designed for the optimized expression of proteins fused to glutathione-S-transferase (GST) in Xenopus laevis oocytes and embryos. The Xenopus model system permits the biochemical analysis of signaling pathways and analysis of embryo phenotype in response to manipulation of proto-oncogene expression. pXen is a modified pSP64T vector which contains an SP6 RNA polymerase promoter...
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