Article
DHFR/MSH3 amplification in methotrexate-resistant cells alters the hMutSalpha/hMutSbeta ratio and reduces the efficiency of base-base mismatch repair.
Proceedings of the National Academy of Sciences of the United States of America - 16 Sept 1997
Drummond J T, Genschel J, Wolf E, Modrich P
Abstract excerpt
The level and fate of hMSH3 (human MutS homolog 3) were examined in the promyelocytic leukemia cell line HL-60 and its methotrexate-resistant derivative HL-60R, which is drug resistant by virtue of an amplification event that spans the dihydrofolate reductase (DHFR) and MSH3 genes. Nuclear extrac...
Topics
- DNA Repair
- DNA-Binding Proteins
- Drug Resistance, Microbial
- Fungal Proteins
- Gene Amplification
- HL-60 Cells
- Humans
- Methotrexate
- Mutation
- Nucleic Acid Heteroduplexes
- Tetrahydrofolate Dehydrogenase