Article
Functional analysis of human MutSalpha and MutSbeta complexes in yeast.
Nucleic acids research - 1 Feb 1999
Clark A B, Cook M E, Tran H T, Gordenin D A, Resnick M A, Kunkel T A
Abstract excerpt
Mismatch repair (MMR) is initiated when a heterodimer of hMSH2*hMSH6 or hMSH2*hMSH3 binds to mismatches. Here we perform functional analyses of these human protein complexes in yeast. We use a sensitive genetic system wherein the rate of single-base deletions in a homopolymeric run in the LYS2 gene is 10 000-fold higher in an msh2 mutant than in a wild-type strain. Expression of the human proteins alone or in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.