Article
The m gamma delta-1 element, a small gamma delta (Tn1000) derivative useful for plasmid mutagenesis, allele replacement and DNA sequencing.
Gene - 1 Apr 1992
Berg C M, Vartak N B, Wang G, Xu X, Liu L, MacNeil D J, Gewain K M, Wiater L A, Berg D E
Abstract excerpt
Transposon gamma delta (Tn1000), a 6-kb member of the Tn3 family, is widely used for plasmid mutagenesis. A 1.8-kb derivative of gamma delta was constructed that contains the kan gene from Tn5 and the resolution (res) site from gamma delta cloned between 40-bp inverted repeats of gamma delta's delta (delta) end. This element, named m gamma delta-1, lacks the genes encoding transposase and resolvase, and therefore...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Genotype
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nucleotidyltransferases