Article
[A simple method of selecting Saccharomyces cerevisiae ade1 and ade2 mutants].
Genetika - 1 Jun 1997
Alenin V V, Getsova M L
Abstract excerpt
A simple technique of selection for spontaneous red mutants of yeast Saccharomyces cerevisiae is proposed. It is based on incubation of yeast cells carrying a deletion in the GCN4 gene on a solid selective medium containing 3-amino-1, 2, 4-triazole (3AT). After incubation for three weeks at 30 degrees C, the 3 AT-resistant mutants ade2 and ade1 were easily visualized due to the pink or red colony color; they were...
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