[Mutation of ade13-1 of the yeast Saccharomyces cerevisiae leads to the absence of growth on a complete medium with glucose and epistatically interacts with mutations in other genes for purine biosynthesis].

Abstract excerpt

Saccharomyces cerevisiae gene ADE13 encodes enzyme adenylosuccinate lyase catalyzing steps 8 and 12 of the de novo purine pathway. The mutation ade13-1 induced by ethyl methanesulfonate leads to a complete loss of enzymatic activity. Stocks with this mutation cannot grow on complete organic media with glucose or fructose as a carbon source, but they can grow on media with glycerol or ethanol. The inhibiting...

Topics

• Adenylosuccinate Lyase
• Cloning, Molecular
• Culture Media
• Genes, Fungal
• Glucose
• Glycolysis
• Mutation
• Purines
• Saccharomyces cerevisiae
• Signal Transduction