Article
[A method of biochemical identification of mutations in red adenine-dependent yeast strains].
Prikladnaia biokhimiia i mikrobiologiia - 1 Jan 2000
Alenin V V, Ostanin K V, Domkin V D
Abstract excerpt
A simple biochemical technique is proposed for quantitative estimation of expression of the ADE2 and ADE1 genes, coding for the structure of AIR-carboxylase and SAICAR-synthetase in the yeast Saccharomyces cerevisiae. The technique is based on determining the enzyme specific activities in the yeast crude extracts. The technique was applied to estimate quantitatively the expression of the ADE2 and ADE1 genes in...
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