Article
Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA function.
Molecular microbiology - 1 Nov 1998
Papavinasasundaram K G, Colston M J, Davis E O
Abstract excerpt
A recA deletion mutant of Mycobacterium smegmatis has been isolated by homologous recombination using a sacB counterselection strategy. Deletion of the recA gene from the chromosome was demonstrated by Southern hybridizations and by polymerase chain reaction (PCR). Western analysis using anti-Rec...
Topics
- Bacterial Proteins
- Gene Deletion
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Mycobacterium smegmatis
- Mycobacterium tuberculosis
- Protein Splicing
- Rec A Recombinases
- Recombination, Genetic
- Restriction Mapping
- Ultraviolet Rays