Article
Disruption of a recombinant yeast for the release of beta-galactosidase.
Bioseparation - 1 Oct 1994
Garrido F, Banerjee U C, Chisti Y, Moo-Young M
Abstract excerpt
A recombinant yeast, Saccharomyces cerevisiae, expressing Escherichia coli beta-galactosidase gene under the control of CYC1 constitutive promoter of the yeast, was disrupted in a continuous flow, high speed, bead mill for the release of intracellular beta-galactosidase (EC 3.2.1.23). Release of the beta-galactosidase activity was characterized with respect to glass bead loading in the grinding chamber (70-85% of...
Topics
- Biotechnology
- Escherichia coli
- Gene Expression Regulation
- Hydrogen-Ion Concentration
- Kinetics
- Microspheres
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- beta-Galactosidase