A new system for the release of heterologous proteins from yeast based on mutant strains deficient in cell integrity.

doi:10.1016/0168-1656(94)90149-x

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Article

A new system for the release of heterologous proteins from yeast based on mutant strains deficient in cell integrity.

Journal of biotechnology - 30 Nov 1994

Alvarez P, Sampedro M, Molina M, Nombela C

Abstract excerpt

A system has been developed for the release of heterologous proteins from Saccharomyces cerevisiae, based on the use of thermosensitive osmotic-remedial mutants, deficient in cell integrity, that lyse at the non-permissive temperature, thus releasing the bulk of intracellular proteins and leaving...

Topics

Chloramphenicol O-Acetyltransferase
Cloning, Molecular
Fungal Proteins
Mitogen-Activated Protein Kinases
Mutation
Osmotic Pressure
Recombinant Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins

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