Article
Improved production of heterologous proteins by a glucose repression-defective mutant of Kluyveromyces lactis.
Applied and environmental microbiology - 1 May 2004
Donnini Claudia, Farina Francesca, Neglia Barbara, Compagno Maria Concetta, Uccelletti Daniela, Goffrini Paola, Palleschi Claudio
Abstract excerpt
The secreted production of heterologous proteins in Kluyveromyces lactis was studied. A glucoamylase (GAA) from the yeast Arxula adeninivorans was used as a reporter protein for the study of the secretion efficiencies of several wild-type and mutant strains of K. lactis. The expression of the reporter protein was placed under the control of the strong promoter of the glyceraldehyde-3-phosphate dehydrogenase of...
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