NMR structural analysis of an analog of an intermediate formed in the rate-determining step of one pathway in the oxidative folding of bovine pancreatic ribonuclease A: automated analysis of 1H, 13C, and 15N resonance assignments for wild-type and [C65S, C72S] mutant forms.

doi:10.1021/bi963024k

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Article

NMR structural analysis of an analog of an intermediate formed in the rate-determining step of one pathway in the oxidative folding of bovine pancreatic ribonuclease A: automated analysis of 1H, 13C, and 15N resonance assignments for wild-type and [C65S, C72S] mutant forms.

Biochemistry - 10 Jun 1997

Shimotakahara S, Rios C B, Laity J H, Zimmerman D E, Scheraga H A, Montelione G T

Abstract excerpt

A three-disulfide intermediate, des-[65-72] RNase A, lacking the disulfide bond between Cys65 and Cys72, is formed in one of the rate-determining steps of the oxidative regeneration pathways of bovine pancreatic ribonuclease A (RNase A). An analog of this intermediate, [C65S, C72S] RNase A, has b...

Topics

Amino Acid Sequence
Animals
Cattle
Crystallography, X-Ray
Disulfides
Hydrogen Bonding
Magnetic Resonance Spectroscopy
Models, Chemical
Models, Molecular
Molecular Sequence Data
Mutation
Oxidation-Reduction
Pancreas

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Protein Folding

Ribonuclease, Pancreatic

Signal Processing, Computer-Assisted

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Source

Open in PubMed

Identifiers

PMID: 9188686
DOI: 10.1021/bi963024k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9188686-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.