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Article

pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate.

Journal of the American Chemical Society - 6 Jul 2011

Du Zhenming, Zheng Yuchuan, Patterson Melissa, Liu Yangzhong, Wang Chunyu

Abstract excerpt

Protein splicing is a robust multistep posttranslational process catalyzed by inteins. In the Mtu RecA intein, a conserved block-F aspartate (D422) coordinates different steps in protein splicing, but the precise mechanism is unclear. Solution NMR shows that D422 has a strikingly high pK(a) of 6.1, two units above the normal pK(a) of aspartate. The elevated pK(a) of D422 is coupled to the depressed pK(a) of...

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Identifiers

PMID: 21604815
DOI: 10.1021/ja203209f
PMCID: PMC3126871

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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