Article
Direct blotting electrophoresis for sequencing and genotyping hepatitis C virus.
Journal of virological methods - 1 May 1997
Castelain S, Khorsi H, Zawadzki P, Sueur J M, Capron J P, Eb F, Duverlie G
Abstract excerpt
Many methods have been used to differentiate the hepatitis C virus (HCV) genotypes based on, for example, type specific primers, probes and restriction fragment length polymorphism. However, determination of the nucleotide sequence remains the reference. Therefore, a simple non-radioactive cycle sequencing technique was developed for clinical tests. PCR-amplified products of the 5' non-coding region (from...
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