Article
Detection and variant identification of HHV-6 by a non-radioactive hybridization microplate assay for amplimers detection.
Journal of virological methods - 26 Apr 1996
Gautheret A, Aubin J T, Poirel L, Chopineau S, Agut H, Dussaix E
Abstract excerpt
A non-radioactive hybridization microtiter plate assay was developed and evaluated for detection of the HHV-6 genome and to identify HHV-6 variants A and B. The viral DNA is amplified by the polymerase chain reaction using a 5'-end-biotinylated primer. The biotinylated amplimers are captured on avidin-coated microtiter plates, denaturated with sodium hydroxide and hybridized to a 3'-end-digoxigenin-labelled...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
