Detection and variant identification of HHV-6 by a non-radioactive hybridization microplate assay for amplimers detection.

doi:10.1016/0166-0934(95)01983-9

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Article

Detection and variant identification of HHV-6 by a non-radioactive hybridization microplate assay for amplimers detection.

Journal of virological methods - 26 Apr 1996

Gautheret A, Aubin J T, Poirel L, Chopineau S, Agut H, Dussaix E

Abstract excerpt

A non-radioactive hybridization microtiter plate assay was developed and evaluated for detection of the HHV-6 genome and to identify HHV-6 variants A and B. The viral DNA is amplified by the polymerase chain reaction using a 5'-end-biotinylated primer. The biotinylated amplimers are captured on a...

Topics

Biotin
Blotting, Southern
Cells, Cultured
DNA Primers
DNA, Viral
Genetic Variation
Herpesviridae Infections
Herpesvirus 6, Human
Humans
Nucleic Acid Hybridization

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