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Isolation of new nonconditional Saccharomyces cerevisiae mutants defective in asparagine-linked glycosylation.

Glycobiology - 1 Jun 1997

Mañas P, Olivero I, Avalos M, Hernández L M

Abstract excerpt

We describe the isolation and partial characterization of Saccharomyces cerevisiae nonconditional mutants that show defects in N-glycosylation of proteins. The selection method is based on the reduction of affinity for the ion exchanger QAE-Sephadex as a consequence of the decrease in the negative charge of the cell surface. This characteristic reflects a decrease in the incorporation of mannosylphosphate units...

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PMID: 9184829
DOI: 10.1093/glycob/7.4.487

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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