Article
Isolation of protein glycosylation mutants in the fission yeast Schizosaccharomyces pombe.
Molecular biology of the cell - 1 May 1995
Huang K M, Snider M D
Abstract excerpt
We have isolated mutants in the fission yeast Schizosaccharomyces pombe that are defective in protein glycosylation. A collection of osmotically sensitive mutants was prepared and screened for glycosylation defects using lectin staining as an assay. Mutants singly defective in four glycoprotein s...
Topics
- Acid Phosphatase
- Cell Wall
- Fungal Proteins
- Galactose
- Genes, Fungal
- Genetic Complementation Test
- Glycosylation
- Lectins
- Mannans
- Membrane Glycoproteins
- Mutation
- Oligosaccharides
- Osmotic Pressure
- Plant Lectins
- Polysaccharides
- Schizosaccharomyces
- UDPglucose 4-Epimerase
- Uridine Diphosphate Galactose