Article
Screening for new yeast mutants affected in mannosylphosphorylation of cell wall mannoproteins.
Yeast (Chichester, England) - 30 Oct 2003
Conde Raúl, Pablo Guadalupe, Cueva Rosario, Larriba Germán
Abstract excerpt
We have carried out a screen of 622 deletion strains generated during the EUROFAN B0 project to identify non-essential genes related to the mannosylphosphate content of the cell wall. By examining the affinity of the deletants for the cationic dye alcian blue and the ion exchanger QAE-Sephadex, we have selected 50 strains. On the basis on their reactivity (blue colour intensity) in the alcian blue assay, mutants...
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