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Article

Single-crossover integration in the Lactobacillus sake chromosome and insertional inactivation of the ptsI and lacL genes.

Applied and environmental microbiology - 1 Jun 1997

Leloup L, Ehrlich S D, Zagorec M, Morel-Deville F

Abstract excerpt

Single-crossover homologous integration in Lactobacillus sake was studied. Integration was conducted with nonreplicative delivery vector pRV300. This vector is composed of a pBluescript SK- replicon for propagation in Escherichia coli and an erythromycin resistance marker. Random chromosomal DNA fragments of L. sake 23K ranging between 0.3 and 3.4 kb were inserted into pRV300. The resulting plasmids were able to...

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Identifiers

PMID: 9172327
DOI: 10.1128/aem.63.6.2117-2123.1997
PMCID: PMC168500

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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