Article
A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica.
Gene - 20 Dec 1991
Kaniga K, Delor I, Cornelis G R
Abstract excerpt
A new suicide vector (pKNG101) that facilitates the positive selection of double recombination events in Gram-bacteria has been developed. It contains a conditional origin of replication (oriR6K), the strAB genes encoding the streptomycin phosphotransferase (SmR), an origin of transfer (mobRK2), the sacB gene mediating sucrose sensitivity, and multiple cloning sites. It was used to mutate the blaA gene of...
Topics
- DNA Replication
- Genetic Vectors
- Gram-Negative Bacteria
- Mutation
- Reading Frames
- Recombination, Genetic
- Yersinia enterocolitica