Article
Expression of Ascaris suum malic enzyme in a mutant Escherichia coli allows production of succinic acid from glucose.
Applied biochemistry and biotechnology - 1 Jan 1997
Stols L, Kulkarni G, Harris B G, Donnelly M I
Abstract excerpt
The malic enzyme gene of Ascaris suum, was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli NZN111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. Induction of pMEA1, which encodes the native animoterminus, gave better overexpression of...
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