Article
A Francisella tularensis DNA clone complements Escherichia coli defective for the production of Era, an essential Ras-like GTP-binding protein.
Gene - 11 Apr 1997
Zuber M, Hoover T A, Dertzbaugh M T, Court D L
Abstract excerpt
We cloned the era gene of Francisella tularensis from a plasmid library by heterologous genetic complementation of an Escherichia coli mutant conditionally defective for the production of Era, an essential protein for cell growth. Nucleotide sequence analysis indicated that, in F. tularensis, era...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Cloning, Molecular
- Conserved Sequence
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Francisella tularensis
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Gene Expression
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- RNA-Binding Proteins
- Tularemia