Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity.

doi:10.1128/jb.177.8.2194-2196.1995

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Article

Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity.

Journal of bacteriology - 1 Apr 1995

Pillutla R C, Sharer J D, Gulati P S, Wu E, Yamashita Y, Lerner C G, Inouye M, March P E

Abstract excerpt

Era is an essential GTP binding protein in Escherichia coli. Two homologs of this protein, Sgp from Streptococcus mutans and Era from Coxiella burnetii, can substitute for the essential function of Era in E. coli. Site-specific and randomly generated Era mutants which may indicate regions of the protein that are of functional importance are described.

Topics

Amino Acid Sequence
Bacterial Proteins
Coxiella burnetii
Escherichia coli
Escherichia coli Proteins
GTP Phosphohydrolases
GTP-Binding Proteins
Genes, Bacterial
Genetic Complementation Test
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Polymerase Chain Reaction

RNA-Binding Proteins

Sequence Homology, Amino Acid

Species Specificity

Streptococcus mutans

Source

Open in PubMed Open full text

Identifiers

PMID: 7721709
DOI: 10.1128/jb.177.8.2194-2196.1995
PMCID: PMC176865

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7721709-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity.PMID 7721709Read ↗

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