Article
Use of the MTT assay in adult ventricular cardiomyocytes to assess viability: effects of adenosine and potassium on cellular survival.
Journal of molecular and cellular cardiology - 1 Apr 1997
Gomez L A, Alekseev A E, Aleksandrova L A, Brady P A, Terzic A
Abstract excerpt
This study used the colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)] assay to assess cell viability in isolated quiescent adult guinea-pig ventricular myocytes exposed to different insults or cardioprotective conditions, including adenosine and hyperkalemic-cardio...
Topics
- Adenosine
- Age Factors
- Animals
- Cardioplegic Solutions
- Cell Adhesion
- Cell Death
- Dimethyl Sulfoxide
- Guinea Pigs
- Heart Ventricles
- Hypoxia
- Nitrogen
- Oxidation-Reduction
- Phenotype
- Potassium
- Spectrophotometry
- Stress, Mechanical
- Tetrazolium Salts