Article
Functional identification of the mouse circadian Clock gene by transgenic BAC rescue.
Cell - 16 May 1997
Antoch M P, Song E J, Chang A M, Vitaterna M H, Zhao Y, Wilsbacher L D, Sangoram A M, King D P, Pinto L H, Takahashi J S
Abstract excerpt
As a complementary approach to positional cloning, we used in vivo complementation with bacterial artificial chromosome (BAC) clones expressed in transgenic mice to identify the circadian Clock gene. A 140 kb BAC transgene completely rescued both the long period and the loss-of-rhythm phenotypes in Clock mutant mice. Analysis with overlapping BAC transgenes demonstrates that a large transcription unit spanning...
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