Article
A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzo[a]pyrene and (+/-)-anti-benzo[a]pyrene-7,8-diol 9,10-oxide in cultured human cells.
Mutation research - 24 Apr 1997
Hanelt S, Helbig R, Hartmann A, Lang M, Seidel A, Speit G
Abstract excerpt
Genotoxic effects of benzo[a]pyrene (BP) and its reactive metabolites (+/-)-anti-benzo[a]pyrene-7,8-diol 9,10-oxide ((+/-)-anti-BPDE) were comparatively investigated in vitro with the permanent human fibroblast cell line MRC5CV1. Induced DNA adducts were measured by 32P-postlabeling, DNA strand breakage was determined by the comet assay and the HPRT gene mutation test was used to detect cytotoxicity and...
Topics
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
- Benzo(a)pyrene
- Cells, Cultured
- DNA
- DNA Adducts
- DNA Damage
- Electrophoresis
- Fibroblasts
- Genetic Techniques
- Humans
- Hypoxanthine Phosphoribosyltransferase