Article
Characterization and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit.
Microbiology (Reading, England) - 1 Apr 1997
Bencina Mojca, Panneman Henk, Ruijter George J G, Legiša Matic, Visser Jaap
Abstract excerpt
The gene pkaC encoding the catalytic subunit of cAMP-dependent protein kinase has been isolated from the industrially important filamentous fungus Aspergillus niger. A probe for screening A. niger phage libraries was generated by a polymerase chain reaction using degenerate primers. cDNA and genomic DNA clones were isolated and sequenced. An open reading frame of 1440 bp, interrupted by three short introns,...
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