Article
Genetic mapping using fluorescent quantification of allele frequencies in pooled DNA loaded by solid support.
Clinical genetics - 1 Mar 1997
Graff C, Persson A, Ulfendahl P J, Wahlberg J, Wadelius C
Abstract excerpt
An efficient and labour-saving method for fragment analysis in linkage studies using biotinylated primers and streptavidin-coated combs is presented. The level of streptavidin attached to the combs was used to control the amount of immobilised material. Thus, the need for titration of PCR products to fit the dynamic range of the sequencer was reduced. The method was used to investigate the possibility of...
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