Article
Multiplex analysis of mutations in four genes using fluorescence scanning technology.
Thrombosis research - 1 Oct 1999
Benson J M, Ellingsen D, Renshaw M A, Resler A G, Evatt B L, Hooper W C
Abstract excerpt
Multiplex analysis of genetic mutations using fluorescence scanning methodology is an accurate, efficient, and cost-effective approach to genotypic characterization. Fluorescence labeling during the synthesis of polymerase chain reaction primers allows the application of this technology to well-established protocols. We have simultaneously analyzed the four polymorphisms of factor V Leiden (G1691A), prothrombin...
Topics
- Cystathionine beta-Synthase
- DNA Mutational Analysis
- DNA Restriction Enzymes
- Electrophoresis, Capillary
- Factor V
- Fluorescent Dyes
- Genotype
- Heterozygote
- Humans
- Methylenetetrahydrofolate Reductase (NADPH2)
- Mutation
- Oxidoreductases Acting on CH-NH Group Donors
- Point Mutation
- Polymerase Chain Reaction
- Prothrombin
- Spectrometry, Fluorescence