Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain.

doi:10.1074/jbc.272.17.11268

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Article

Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain.

The Journal of biological chemistry - 25 Apr 1997

Elce J S, Hegadorn C, Arthur J S

Abstract excerpt

The roles of N-terminal autolysis of the large (80 kDa) and small (28 kDa) subunits in activation of rat m-calpain, in lowering its Ca2+ requirement, and in reducing its stability have been investigated with heterodimeric recombinant calpains containing modified subunits. Both autolysis and [Ca2+...

Topics

Animals
Autolysis
Calcium
Calpain
Caseins
Dimerization
Enzyme Stability
Mutation
Protein Conformation
Rats
Recombinant Proteins
Sequence Deletion

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