Article
Roles of individual EF-hands in the activation of m-calpain by calcium.
The Biochemical journal - 15 May 2000
Dutt P, Arthur J S, Grochulski P, Cygler M, Elce J S
Abstract excerpt
m-Calpain is a heterodimeric, cytosolic, thiol protease, which is activated by Ca(2+)-binding to EF-hands in the C-terminal domains of both subunits. There are four potential Ca(2+)-binding EF-hands in each subunit, but their relative affinities for Ca(2+) are not known. In the present study mutations were made in both subunits to reduce the Ca(2+)-binding affinity at one or more EF-hands in one or both subunits....
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