Article
Characterization of Hprt mutations in cDNA and genomic DNA of T-cell mutants from control and 1,3-butadiene-exposed male B6C3F1 mice and F344 rats.
Environmental and molecular mutagenesis - 1 Jan 2004
Meng Quanxin, Walker Dale M, Scott Bobby R, Seilkop Steve K, Aden James K, Walker Vernon E
Abstract excerpt
A multiplex PCR procedure for analysis of genomic DNA mutations in the mouse hypoxanthine-guanine phosphoribosyltransferase (Hprt) gene was developed and then used with other established methods for the coincident identification of large- and small-scale genetic alterations in the Hprt gene of mutant T-cell isolates propagated from sham- and 1,3-butadiene (BD)-exposed mice and rats. The spectra data for...
Topics
- Animals
- Butadienes
- DNA
- DNA Mutational Analysis
- DNA, Complementary
- Hypoxanthine Phosphoribosyltransferase
- Male
- Mice
- Mice, Inbred Strains
- Mutagens
- Mutation
- Rats
- Rats, Inbred F344
- Reverse Transcriptase Polymerase Chain Reaction