Article
A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing.
Genetic testing - 1 Jan 2006
Downing J, Coates E, Street J, Hammond L, Rees T J, Pepperall J, Darke C
Abstract excerpt
We designed a set of 35 polymerase chain reaction sequence-specific primers (PCR-SSP) in 29 SSP mixtures to assign 29 HLA-B*27 4-digit level alleles (B*2701-B*2721 and B*2723-B*2730). This was used in conjunction with our 41 PCR-SSP primer mixture low-resolution HLA-B typing set to fully differentiate B*27 from all other HLA-B alleles. Successful typing set validation used 521 B*27 samples covering 13...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
