Article
Molecular typing of HLA-B27 alleles.
Immunogenetics - 1 Jan 1992
Dominguez O, Coto E, Martinez-Naves E, Choo S Y, López-Larrea C
Abstract excerpt
HLA-B27 represents a family of closely related antigens. Six alleles which differ in a limited number of nucleotide substitutions have been described (B*2701--B*2706). These changes are clustered in alpha 1 and alpha 2 domains. Polymerase chain reaction strategies were designed to amplify specific regions of class I exons 2 and 3. Amplified sequences were tested with eight sequence-specific oligonucleotides to...
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