Article
Temperature-sensitive mutants of Corynebacterium ammoniagenes ATCC 6872 with a defective large subunit of the manganese-containing ribonucleotide reductase.
Archives of microbiology - 1 May 1997
Luo C, Hansen J, Auling G
Abstract excerpt
Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRas...
Topics
- Brevibacterium
- Corynebacterium
- DNA Replication
- Escherichia coli
- Genetic Complementation Test
- Manganese
- Microscopy, Electron, Scanning
- Mutagenesis
- Mutation
- Protein Conformation
- Ribonucleotide Reductases
- Temperature