Article
Probing the Sinorhizobium meliloti-alfalfa symbiosis using temperature-sensitive and impaired-function citrate synthase mutants.
Molecular plant-microbe interactions : MPMI - 1 Feb 2005
Grzemski Wojciech, Akowski James P, Kahn Michael L
Abstract excerpt
To study the role of the decarboxylating leg of the bacterial TCA cycle in symbiotic nitrogen fixation, we used DNA shuffling and localized random polymerase chain reaction mutagenesis to construct a series of temperature-sensitive and impaired-function mutants in the Sinorhizobium meliloti Rm104...
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