Article
Characterisation of D117A and H260A mutations in the melanocortin 1 receptor.
Molecular and cellular endocrinology - 7 Feb 1997
Schiöth H B, Muceniece R, Szardenings M, Prusis P, Lindeberg G, Sharma S D, Hruby V J, Wikberg J E
Abstract excerpt
Recent site directed mutagenesis studies on the melanocortin 1 (MC1) receptor have indicated the importance of D117 and H260 amino acid residues for the binding of alpha-MSH (melanocyte stimulating hormone). Here, we report the testing of 12 cyclic and linear MSH peptides on the D117A and H260A mutant receptors. Moreover, we constructed a double mutant which displayed a major loss in affinity for [Nle4,...
Topics
- Animals
- COS Cells
- Cloning, Molecular
- Humans
- Mutagenesis
- Mutation
- Receptors, Corticotropin
- Receptors, Melanocortin
- Transfection