Article
Rapid plasma membrane anchoring of newly synthesized p59fyn: selective requirement for NH2-terminal myristoylation and palmitoylation at cysteine-3.
The Journal of cell biology - 10 Mar 1997
van't Hof W, Resh M D
Abstract excerpt
The trafficking of Src family proteins after biosynthesis is poorly defined. Here we studied the role of dual fatty acylation with myristate and palmitate in biosynthetic transport of p59fyn. Metabolic labeling of transfected COS or NIH 3T3 cells with [35S]methionine followed by analysis of cytos...
Topics
- 3T3 Cells
- Acylation
- Animals
- COS Cells
- Cell Membrane
- Cysteine
- Detergents
- GAP-43 Protein
- GTP-Binding Proteins
- Humans
- Membrane Glycoproteins
- Mice
- Mutation