Article
Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporter.
Biochimica et biophysica acta - 21 Feb 1997
Kasahara T, Kasahara M
Abstract excerpt
Rat Glut4 glucose transporter was expressed in the yeast Saccharomyces cerevisiae, but was retained in an intracellular membranous compartment and did not contribute to glucose uptake by intact cells. A crude membrane fraction was prepared and reconstituted in liposome with the use of the freeze-thaw/sonication method. D-glucose-specific, cytochalasin B inhibitable glucose transport activity was observed. Kinetic...
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