Article
Functional asymmetry of the sodium-D-glucose cotransporter expressed in yeast secretory vesicles.
The Journal of membrane biology - 15 Jan 2001
Firnges M A, Lin J T, Kinne R K
Abstract excerpt
The sodium-D-glucose cotransporter (SGLT1) was expressed in a yeast mutant strain NY 17 (sec6-4) that accumulates secretory vesicles at a nonpermissive temperature because of a block in the delivery of these vesicles to the plasma membrane. By differential centrifugation a microsomal fraction enriched in secretory vesicles was prepared with a high specific activity of the vanadate-sensitive H+-ATPase and...
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