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Article

Transformation and strain engineering of Tetrahymena.

Methods in cell biology - 1 Jan 2012

Chalker Douglas L

Abstract excerpt

Transformation of Tetrahymena by microinjection of DNA was established 25 years ago. This rather labor-intensive technique has since been shelved, replaced by less time consuming and more efficient methods, electroporation and biolistics. Conjugative electroporation is the method of choice for introducing autonomously replicating, rDNA-based vectors into Tetrahymena. These are maintained as high-copy linear...

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PMID: 22444150
DOI: 10.1016/B978-0-12-385967-9.00011-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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