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Article

Manipulating ciliary protein-encoding genes in Tetrahymena thermophila.

Methods in cell biology - 1 Jan 2009

Dave Drashti, Wloga Dorota, Gaertig Jacek

Abstract excerpt

Tetrahymena thermophila has emerged as an excellent protist model for studies on cilia that are based on reverse genetic approaches. In Tetrahymena, genes can be routinely disrupted by the DNA homologous recombination. We present established protocols for the manipulation of genes in either the germline micronucleus or the somatic macronucleus. A detailed protocol is provided for the construction of heterokaryon...

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PMID: 20409809
DOI: 10.1016/S0091-679X(08)93001-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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