Article
Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.
Genetics - 1 Dec 1992
Cole E S, Bruns P J
Abstract excerpt
A new method of inducing self-fertilization, uniparental cytogamy, yields homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. Progeny are highly fertile and show a marked tendency for precocious sexual maturity. This method is highly effective in protocols designed to generate and express nonlethal dominant or recessive mutations.
Topics
- Animals
- Cell Nucleus
- Culture Media
- Drug Resistance
- Gene Expression
- Homozygote
- Mutagenesis
- Mutation
- Osmolar Concentration
- Ploidies
- Tetrahymena thermophila
- Time Factors