Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An efficient laboratory protocol for the sequencing of large numbers of lacI mutants recovered from Big Blue transgenic animals.

Environmental and molecular mutagenesis - 1 Jan 1996

Erfle H L, Walsh D F, Holcroft J, Hague N, de Boer J G, Glickman B W

Abstract excerpt

Mutational spectra provide a powerful approach to investigate both the mutagenic potential and the mechanism of action of suspected mutagens and carcinogens. Recently, transgenic techniques have made it possible to generate mutational spectra in animals. Such a spectrum may consist of 50 to 200 mutants depending on the nature of the mutations, and many spectra can be generated depending on the design of the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mice, Transgenic

Share to Mutation

Share to Polymerase Chain Reaction

Share to Sequence Analysis, DNA

Source

Identifiers

PMID: 8991068
DOI: 10.1002/(SICI)1098-2280(1996)28:4<393::AID-EM13>3.0.CO;2-A

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An efficient laboratory protocol for the sequencing of large numbers of lacI mutants recovered from Big Blue transgenic animals.PMID 8991068Read ↗

Software package for the management of sequencing projects using lacI transgenic animals.Semantic proximity Read ↗

The lacI gene as a target for mutation in transgenic rodents and Escherichia coli.Semantic proximity Read ↗

Spectrum of spontaneous mutations in liver tissue of lacI transgenic mice.Semantic proximity Read ↗

LacI mutation spectra following benzo[a]pyrene treatment of Big Blue mice.Semantic proximity Read ↗

Spontaneous mutations in the Big Blue transgenic system are primarily mouse derived.Semantic proximity Read ↗

Database and software for the analysis of mutations at the lacI gene in both transgenic rodents and bacteria.Semantic proximity Read ↗

On the origin of unusual mutations recovered from the lacI transgenic assay.Semantic proximity Read ↗

Spontaneous mutation in lacI transgenic mice: a comparison of tissues.Semantic proximity Read ↗

Towards validation of the Big Blue transgenic mouse mutagenesis assay: the mutational spectrum of ex vivo pinpoint mutant plaques.Semantic proximity Read ↗

A more efficient Big Blue protocol improves transgene rescue and accuracy in a adduct and mutation measurement.Semantic proximity Read ↗

Spontaneous mutation frequencies and spectra in p53 (+/+) and p53 (-/-) mice: a test of the 'guardian of the genome' hypothesis in the Big Blue transgenic mouse mutation detection system.Semantic proximity Read ↗

Spontaneous mutation in Big Blue transgenic mice: analysis of age, gender, and tissue type.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.